Guest post from Student Alex Martin on Kittybiome & Animal Shelters

We are nearing the end of our Kitty Kickstarter to fund research on the microbiome of cats (only three days left).  We have received some requests to learn more about our work with animal shelters. Here is a blog post by Alex Martin, a UC Berkeley junior who is working with us to study shelter cats in Berkeley.

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The Berkeley Animal Shelter takes in all cats from within Berkeley city limits. Thus, cats who once varied markedly with regards to diet and home environment come to live under a fairly uniform set of conditions. It typically houses between fifteen and forty cats, but has held as many as seventy during the peak of breeding season. Recently we have begun collecting samples from cats at the Berkeley shelter in order to better understand their gut microbiomes.

A major dichotomy in the shelter cat population is the one separating house cats from feral cats. Both are considered domestic cats, members of the species Felis catus. If a kitten during its first few months of life is not exposed to humans, it develops behaviors to facilitate surviving in the wild, and grows up to become a feral cat. Some see feral cats as a nuisance, but the animals also tend to live difficult lives, enduring food shortages and a lack of medical care. Thus, a relatively new effort referred to as “trap-neuter-return”(TNR) aims to spay and neuter feral cats to slowly and humanely diminish the size and number of feral cat colonies. Differences in the gut microbiomes of feral cats versus their tamer counterparts is perhaps expected, as the two groups have vastly different diets and levels of environmental exposure. However, these differences have yet to be characterized.

In addition to the differences between feral and house cats, a small but important FIV(Feline Immunodeficiency Virus) population can potentially serve as an interesting point of comparison. Much like Human Immunodeficiency Virus, FIV attacks the immune system of infected individuals, making them markedly more susceptible to other infections. We think that this virus will affect the microbiome of FIV-positive cats in measurable ways. By identifying any differences, we will gain a better understanding of FIV as a whole and will hopefully be better positioned to one day develop more effective methods of treatment.

Geronimo is one Berkeley shelter cat whose gut microbiome will be analyzed. He was picked up as a stray just a few blocks from the shelter, and is three years old. Geronimo is exceptionally friendly, and loves playing with his wand toy and hiding in his cat tree to nap. He gets along well with other cats and was even introduced to a rabbit without incident. After spending about two weeks in the shelter, Geronimo was adopted into a loving home.

Guest post on Yet Another Mostly Male Meeting (YAMMM) - Programming for Biology

I have posted on Twitter and other places saying that I would be willing to share here anonymous postings about meetings with skewed gender ratios.  I generally am not overly fond on anonymity on the web but realize it has some very important and powerful uses, including protecting people from retribution.  So in the case of meetings with skewed gender ratios, I know from personal experience that posting about them can lead to serious vitriol, threats, and possible repercussions.  I feel confident enough in my status and position to mostly ignore these responses but I know that not everyone is so blessed.

So - here is one such anonymous post I received.
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Last year I was looking around for good workshops to learn programming for biology
(http://programmingforbiology.org/index.html) and
about genome assembly and annotation. I came across the Cold Spring Harbor Laboratory
course called "Programming for Biology" and applied as they have a good reputation.
I was happy to get in and overall really enjoyed the course. I learnt how to program in
Perl (not Python what I regret a bit), a lot of background on downstream genome analysis
and had a mostly pleasant time. An interesting slogan of the meeting was
"It's not only what you learn here, but also who you meet that makes this workshop so special"
(I am paraphrasing here a bit). Great! But wait are all big players in the field of bioinformatics
guys?

Out of the 10 Guest Lectures ALL were male.

• Scott Cain             Ontario Institute for Cancer Research
• Brian Haas            Broad Institute
• Winston Hide        Harvard School of Public Health, South African National Bioinformatics Institute
• Tomas Marques     Universitat Pompeu Fabra
• Barry Moore          University of Utah
• William Pearson    University of Virginia
• James Robinson     Broad Institute
• Michael Schatz      CSHL
• Jason Stajich          University of California, Riverside
• Paul Thomas          University of Southern California

Only 2 out of the 7 instructors and tutors were female

• Simon Prochnik DOE - Joint Genome Institute, Walnut Creek, CA
• Sofia Robb         University of California, Riverside
• Steven Ahrendt University of California, Riverside
• Dave Messina Cofactor Genomics
• Shawn Rynearson University of Utah
• Deborah Triant University of Virginia
• Ken Youens-Clark University of Arizona

This means only 2 out of 17 teachers were actual women. This together with the fact that
the meeting was also sold as 'who you meet here is important' was the most disappointing
fact. There are so many talented and great female bioinformatics out there it would be
great to see at least some of them present at this workshop in 2015.

P.S.: Don't get me wrong Simon and Sofia are great and organize a lovely meeting. So this
goes under the category 'even when it hurts'.

Holly Ganz @hollyhganz on Why She Started the @Kittybiome Cat Microbiome Project

The Story Behind the Launch of the Kittybiome Cat Microbiome Project

Guest Post by Holly Ganz (Project Scientist in the Eisen Lab)



Recently a group of us launched a Kitty Kickstarter campaign where we offer to sequence the bacteria in your cat’s gut microbiome as part of a long term research project to learn more about how microbes may affect cat health, behavior and evolutionary history (and vice-versa). Jonathan has written about the origins story here. This project complements our interests in the microbiology of animal shelters and the evolution of bacterial communities in the Felidae. In addition, we thought that other people like cats too and might be interested in learning more about the hidden life of their cats. We have had an overwhelming response that vastly exceeded our expectations (and we are still welcoming more kitty “pawticipants”).



We have been asked “Why cats?” Personally I think it’s hard not to be fanatical about cats. The diversity of cats is astonishing and most people agree that cheetahs, leopards, lions and tigers are amazing. And when you see a lion in the wild for the first time, it’s hard not to see some of your house cat in there, in the way that it walks, naps, yawns, and even pounces (but not so much the roar). Also it’s really interesting that domestic cats have been associated with people for something like 10,000 years. Several years ago I decided to take what I learned from studying microbial ecology in soil (and how it might affect the transmission of anthrax in zebras) and apply it towards understanding the microbial ecology of the animals themselves. I believe that research in the microbiome of cats (and humans) will eventually lead to useful interventions.

In our kittybiome project, we aim to sequence the gut microbiome of 1,000 cats and by doing so begin to capture the variation in gut bacteria in different populations of cats (both domestic and wild). In domestic cats, we will compare cats living in houses with cats living in shelters and feral cats. We are starting to compare cats with different health conditions and have had some cats with diabetes and IBD join the project. This aspect of the project is really important because these conditions are fairly common and there is a lot of room for improvement in how cats suffering from IBD in particular are treated.



We are also collaborating with Adrian Tordiffe at the University of Pretoria, South Africa and the Africat Foundation on a study on how the diet of captive cheetahs might affect the gut microbiome. Here we hypothesize that the unnatural diet of captive cheetahs produces changes in the gut microbiota that may result in some of chronic diseases common in captive cheetahs.




In addition to being fanatical about cats and passionate about poop, I am especially interested in how social behavior affects the composition and function of microbial communities in cats (in their poop and their anal glands!). (My life was changed by reading about hyena scent gland bacteria.) The evolution of the interaction between cats and their symbiotic scent gland bacteria fascinates me. In the Serengeti, residential territorial cheetahs have been observed scent marking on an hourly basis. Domestic cats are really interesting because feral cats form social colonies. The only other cats that are social are lions (who form prides) and cheetahs (who form coalitions). We are comparing these cats with some social structure with some of their close relatives who are solitary (black-footed cats, leopards, and pumas).

Guest Post: 5 Things You Probably Didn't Know About Jonathan Eisen

5 Things You Probably Didn't Know About Jonathan Eisen 

1. He doesn't know how to play Minecraft
2. He mailed grass when he was a little kid
3. His new phone is "precious" to him
4. He loves Let it Go and Taylor Swift
5. He has very ugly pink boots 

by his bored daughter A. I. Eisen

Overselling the mcirobiome award: Dr Roizen's Preventative and Integrative Medicine Conference

Just got an email announcement for "Dr. Roizen's Preventative and Integrative Medicine Conference" in Las Vegas in December 2015.

The announcement did not start of well for me with the gender balance of the key speakers

But since I spoke at this meeting in 2013 and since there was a good gender balance at that meeting, I decided to give the benefit of the doubt and keep reading (though I note - not trying to say this 5:0 gender ratio is a good thing).

And this is when it got worse - here are the bullet points for what one should learn from attending this meeting

• The key concept about optimal aging that Dr. Roizen learned from 56 million people who took the RealAge® test
• Smart tips about changing you and your patient's microbiomes and what to do for your microbiome to promote weight loss and how it inhibits aging
• How you can affect the role of the GI tract in chronic disease
• How to understand the clinical utility of TMAO testing for monitoring cardiometabolic risk
• The tricks about measuring your microbiome's effects
• Why some choose a plant based diet and why you might not
• What supplements do you and your patient's need with a plant based diet to decrease inflammation and improve your microbiome
• Clarify how a systems-based approach can effectively treat illness and promote wellness

Now - I don't know much about Dr. Roizen or his optimal aging claims in his books (I am skeptical). But the microbiome stuff in here is silly.

Let's start with: "Smart tips about changing you and your patient's microbiomes and what to do for your microbiome to promote weight loss and how it inhibits aging".  I wonder how he will give these smart tips when as far as I know there is nothing actually known about this.  How the microbiome inhibits aging?  Really? Is this going to be a summary of future research not yet done or even imagined?

What about "The tricks about measuring your microbiome's effects."  So - there are 1000s of scientists studying this, they mostly say it is very very very hard to study the effects of the microbiome and Roizen and crew are going to solve this with a few "tricks"?  So is he saying everyone in the field is incompetent since they can't measure these effects but he knows how to with a few tricks?

Dr. Roizen seems like a smart person and some of what I have heard from him sounds reasonable.  These microbiome claims from him here are a clear example of "Overselling the microbiome" and buying into the hype and not staying with the science. Maybe he was not paying attention for my talk for this meeting in 2013 when I discussed overselling the microbiome

I hope he tones down his claims in the future ... but for now he is a winner of a coveted "Overselling the Microbiome Award".  For other "winners" see here.

Blind trust in unblinded observation in Ecology, Evolution and Behavior (Guest Post by Melissa Kardish)

This is a guest post from Melissa Kardish - a PhD student at UC Davis - writing about a recent paper from work she did at her prior position.  The citation for the paper she is writing about is below:

Kardish MR, Mueller UG, Amador-Vargas S, Dietrich EI, Ma R, Barrett B and Fang C-C (2015) Blind trust in unblinded observation in Ecology, Evolution, and Behavior. Front. Ecol. Evol. 3:51. doi: 10.3389/fevo.2015.00051

Here is her post.

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Blind trust in unblinded observation in Ecology, Evolution and Behavior

We recently published our study in Frontiers in Ecology and Evolution where we found that a remarkable number of studies that could be affected by observer bias didn’t indicate whether or not they blinded their research. In fact only 13.3% of studies reported this:

We tried to make this a very transparent study. In addition to journal level data in the main article, we include in our supplemental material a table with the score for every article we read for this study (a summary of these scores per journal can be found in Figure S2 included here). If anything, our results under-represent the amount of studies that could have been scored blind (the real underreporting/underuse of blind observation is probably less than the 13.3% we report). For instance, we did not assess that there was potential for bias in the scoring of microsatellite markers (scored as unlikely to have observer bias). However, we did identify one study which was based on data from microsatellites which did blindly score their markers and report this scoring in their methods (and was therefore scored as “blind” in our study).  We also considered a study blind in its entirety for the purposes of our scoring if only one aspect is reported even if other experiments could also have been influenced by observer bias (Check out our supplemental methods for more ways we conservatively scored in our study).

We recognize that not all EEB studies can be blinded due to a variety of logistical or hypothesis driven reasons; however, we encourage such studies to accurately report this rationale and consider and attempt to minimize observer bias when designing experiments.

Thus far we have had a great response from the surveyed journals. Many of them have notified their editors about the lack of blind observation that we found reported in their journal. One journal has even notified us of plans already in place to address this issue at their next editorial board meeting.

We’re excited to have this work out there and hope this will inspire people to blind their studies and accurately report the science they are doing. We’re also excited to have the study published in an open-access format where we hope the encouragement for blind observation can reach all levels of science. Finally, as reporting of science in our fields improves in the coming years, we hope this study can serve as a template to address other potential concerns in experimental design and reporting.

For our article check out:  http://journal.frontiersin.org/article/10.3389/fevo.2015.00051/full

For our supplemental text and methods: http://journal.frontiersin.org/file/downloadfile/10238/octet-stream/Data%20Sheet%201.PDF/711/2/142945

For our data table with each of the articles we surveyed: http://journal.frontiersin.org/file/downloadfile/10235/octet-stream/Table%201.XLSX/711/1/142945

Nice story from Dan Potter on KQED about Women Science PhDs

Nice story on KQED from Dan Potter: Women Getting Science Ph.D.s Still Face Gender Barriers